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facs buffer  (Bio-Rad)


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    Structured Review

    Bio-Rad facs buffer
    Facs Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 743 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/facs+buffer/Mouse+anti+V5-Tag/bio_rxiv__64898__2026__04__27__720530-146-14-23
    Average 96 stars, based on 743 article reviews
    facs buffer - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Human iPSC-derived macrophages for studying intrinsic and extrinsic factors in cystic fibrosis
    Article Snippet: After this, iMacs were washed with basal media, and collected using TrypLE Express (Gibco) for 10 minutes. .. Cells were washed and stained at 1:100 for BV421-CD11b (BioLegend), washed twice in FACS buffer (2% FBS and 0.5 mM EDTA in 1X PBS), and analyzed on a BioRad ZE5 cytometer (Columbia CSCI Flow Cytometry core). .. LM2 subclones of MDA-MB-231 breast cancer cells (provided by the Massagué lab at MSKCC) were collected with TrypLE Express dissociation solution for 5 minutes, washed, and incubated with CFSE solution (Abcam) for 20 minutes at 37 °C.

    Article Title: Microglia modulate the cerebrovascular reactivity through ectonucleotidase CD39
    Article Snippet: Tissue was rapidly dissected and mechanically disssociated in HBSS and centrifuged over 20% Percoll (GE Healthcare) and mononuclear cells were collected. .. Cells were resuspended in FACS buffer (500mL sterile PBS, .5% BSA, 2ml 0.5M EDTA), blocked in FC blocker (BioRad) as per manufacturer’s instructions and then stained on ice for 15 minutes with a combination of the following antibodies: CD73-PE (12-0731-82, ThermoFisher), CD39-Alexafluor700 (24DMS1, ThermoFisher). ..

    Article Title: Multi-dimensional optimization of a lysin towards a ribolysin against life-threatening S. aureus infections: Fc-LysM-CHAP and its strong synergy with standard of care antibiotics
    Article Snippet: .. After the third wash, cells were adjusted to an OD 600nm of 1.0 in FACS buffer and stained with 1 μg/mL anti-V5-Alexa647 (MCA1360A647, Bio-Rad) for 1 hour while rotating protected from light at 4°C. ..

    FACS:

    Article Title: Human iPSC-derived macrophages for studying intrinsic and extrinsic factors in cystic fibrosis
    Article Snippet: After this, iMacs were washed with basal media, and collected using TrypLE Express (Gibco) for 10 minutes. .. Cells were washed and stained at 1:100 for BV421-CD11b (BioLegend), washed twice in FACS buffer (2% FBS and 0.5 mM EDTA in 1X PBS), and analyzed on a BioRad ZE5 cytometer (Columbia CSCI Flow Cytometry core). .. LM2 subclones of MDA-MB-231 breast cancer cells (provided by the Massagué lab at MSKCC) were collected with TrypLE Express dissociation solution for 5 minutes, washed, and incubated with CFSE solution (Abcam) for 20 minutes at 37 °C.

    Article Title: Sequential platinum and PARP Inhibition enhances PD1 immunotherapy efficacy in murine Brca2 mutated pancreatic cancer
    Article Snippet: .. Tissue suspensions were filtered through a 70-μm nylon strainer (Corning) before being incubated in ACK lysing buffer (Life Technologies) for 5 min, washed with FACS buffer (PBS, 2% FBS and 2 mM EDTA) and counted using a TC20 automated cell counter (Bio-Rad). .. Single cell suspensions were stained with Aqua dead cell stain kit (Life Technologies) for 20 min at room temperature followed by a master mix of primary conjugated antibodies (Supplementary Table S2) for 30 min at 4 °C.

    Article Title: MNT: a new target for AML
    Article Snippet: .. Cellular composition of haemopoietic tissues was determined by flow cytometry: single cell suspensions of bone marrow or spleen were prepared in FACS buffer (phosphate-buffered saline (PBS) supplemented with 1% heat inactivated foetal bovine serum (HI-FBS) (A4503, Sigma-Aldrich)) and cells were counted using the Bio-Rad TC20 automated cell counter (BioRad Laboratories, Inc., Hercules, California, United States). ..

    Article Title: Sequential platinum and PARP Inhibition enhances PD1 immunotherapy efficacy in murine Brca2 mutated pancreatic cancer.
    Article Snippet: .. Tissue suspensions were filtered 194 through a 70-μm nylon strainer (Corning) before being incubated in ACK lysing 195 buffer (Life Technologies) for 5 minutes, washed with FACS buffer (PBS, 2% FBS 196 and 2 mM EDTA) and counted using a TC20 automated cell counter (Bio-Rad). .. Single 197 cell suspensions were stained with Aqua dead cell stain kit (Life Technologies) for 198 20 minutes at room temperature followed by a master mix of primary conjugated 199 antibodies (Supplementary Table S2) for 30 minutes at 4°C.

    Article Title: Microglia modulate the cerebrovascular reactivity through ectonucleotidase CD39
    Article Snippet: Tissue was rapidly dissected and mechanically disssociated in HBSS and centrifuged over 20% Percoll (GE Healthcare) and mononuclear cells were collected. .. Cells were resuspended in FACS buffer (500mL sterile PBS, .5% BSA, 2ml 0.5M EDTA), blocked in FC blocker (BioRad) as per manufacturer’s instructions and then stained on ice for 15 minutes with a combination of the following antibodies: CD73-PE (12-0731-82, ThermoFisher), CD39-Alexafluor700 (24DMS1, ThermoFisher). ..

    Article Title: Multi-dimensional optimization of a lysin towards a ribolysin against life-threatening S. aureus infections: Fc-LysM-CHAP and its strong synergy with standard of care antibiotics
    Article Snippet: .. After the third wash, cells were adjusted to an OD 600nm of 1.0 in FACS buffer and stained with 1 μg/mL anti-V5-Alexa647 (MCA1360A647, Bio-Rad) for 1 hour while rotating protected from light at 4°C. ..

    Cytometry:

    Article Title: Human iPSC-derived macrophages for studying intrinsic and extrinsic factors in cystic fibrosis
    Article Snippet: After this, iMacs were washed with basal media, and collected using TrypLE Express (Gibco) for 10 minutes. .. Cells were washed and stained at 1:100 for BV421-CD11b (BioLegend), washed twice in FACS buffer (2% FBS and 0.5 mM EDTA in 1X PBS), and analyzed on a BioRad ZE5 cytometer (Columbia CSCI Flow Cytometry core). .. LM2 subclones of MDA-MB-231 breast cancer cells (provided by the Massagué lab at MSKCC) were collected with TrypLE Express dissociation solution for 5 minutes, washed, and incubated with CFSE solution (Abcam) for 20 minutes at 37 °C.

    Flow Cytometry:

    Article Title: Human iPSC-derived macrophages for studying intrinsic and extrinsic factors in cystic fibrosis
    Article Snippet: After this, iMacs were washed with basal media, and collected using TrypLE Express (Gibco) for 10 minutes. .. Cells were washed and stained at 1:100 for BV421-CD11b (BioLegend), washed twice in FACS buffer (2% FBS and 0.5 mM EDTA in 1X PBS), and analyzed on a BioRad ZE5 cytometer (Columbia CSCI Flow Cytometry core). .. LM2 subclones of MDA-MB-231 breast cancer cells (provided by the Massagué lab at MSKCC) were collected with TrypLE Express dissociation solution for 5 minutes, washed, and incubated with CFSE solution (Abcam) for 20 minutes at 37 °C.

    Article Title: MNT: a new target for AML
    Article Snippet: .. Cellular composition of haemopoietic tissues was determined by flow cytometry: single cell suspensions of bone marrow or spleen were prepared in FACS buffer (phosphate-buffered saline (PBS) supplemented with 1% heat inactivated foetal bovine serum (HI-FBS) (A4503, Sigma-Aldrich)) and cells were counted using the Bio-Rad TC20 automated cell counter (BioRad Laboratories, Inc., Hercules, California, United States). ..

    Incubation:

    Article Title: Sequential platinum and PARP Inhibition enhances PD1 immunotherapy efficacy in murine Brca2 mutated pancreatic cancer
    Article Snippet: .. Tissue suspensions were filtered through a 70-μm nylon strainer (Corning) before being incubated in ACK lysing buffer (Life Technologies) for 5 min, washed with FACS buffer (PBS, 2% FBS and 2 mM EDTA) and counted using a TC20 automated cell counter (Bio-Rad). .. Single cell suspensions were stained with Aqua dead cell stain kit (Life Technologies) for 20 min at room temperature followed by a master mix of primary conjugated antibodies (Supplementary Table S2) for 30 min at 4 °C.

    Article Title: Sequential platinum and PARP Inhibition enhances PD1 immunotherapy efficacy in murine Brca2 mutated pancreatic cancer.
    Article Snippet: .. Tissue suspensions were filtered 194 through a 70-μm nylon strainer (Corning) before being incubated in ACK lysing 195 buffer (Life Technologies) for 5 minutes, washed with FACS buffer (PBS, 2% FBS 196 and 2 mM EDTA) and counted using a TC20 automated cell counter (Bio-Rad). .. Single 197 cell suspensions were stained with Aqua dead cell stain kit (Life Technologies) for 198 20 minutes at room temperature followed by a master mix of primary conjugated 199 antibodies (Supplementary Table S2) for 30 minutes at 4°C.

    Single Cell:

    Article Title: MNT: a new target for AML
    Article Snippet: .. Cellular composition of haemopoietic tissues was determined by flow cytometry: single cell suspensions of bone marrow or spleen were prepared in FACS buffer (phosphate-buffered saline (PBS) supplemented with 1% heat inactivated foetal bovine serum (HI-FBS) (A4503, Sigma-Aldrich)) and cells were counted using the Bio-Rad TC20 automated cell counter (BioRad Laboratories, Inc., Hercules, California, United States). ..

    Saline:

    Article Title: MNT: a new target for AML
    Article Snippet: .. Cellular composition of haemopoietic tissues was determined by flow cytometry: single cell suspensions of bone marrow or spleen were prepared in FACS buffer (phosphate-buffered saline (PBS) supplemented with 1% heat inactivated foetal bovine serum (HI-FBS) (A4503, Sigma-Aldrich)) and cells were counted using the Bio-Rad TC20 automated cell counter (BioRad Laboratories, Inc., Hercules, California, United States). ..

    Sterility:

    Article Title: Microglia modulate the cerebrovascular reactivity through ectonucleotidase CD39
    Article Snippet: Tissue was rapidly dissected and mechanically disssociated in HBSS and centrifuged over 20% Percoll (GE Healthcare) and mononuclear cells were collected. .. Cells were resuspended in FACS buffer (500mL sterile PBS, .5% BSA, 2ml 0.5M EDTA), blocked in FC blocker (BioRad) as per manufacturer’s instructions and then stained on ice for 15 minutes with a combination of the following antibodies: CD73-PE (12-0731-82, ThermoFisher), CD39-Alexafluor700 (24DMS1, ThermoFisher). ..

    other:

    Article Title: PEPITEM Regulates the Synovial Microenvironment During Immune-Mediated Inflammatory Arthritis to Limit Disease.
    Article Snippet: For collagen-induced arthritis (CIA) and antigen-induced arthritis (AIA), isolated joints were enzymatically digested for 45 minutes at 37 C in collagenase D (0.1 g/mL, Roche) and DNase I (0.01 g/mL, Sigma-Aldrich) diluted in RPMI1640 containing 2% fetal calf serum (Biosera) (step 1), before filtering using 100-μm cell PEPITEM MODULATES THE SYNOVIAL NICHE 5 23265205, 0, A SP - N E PA L , on [14/04/2026]

    Article Title: ALS/FTD-linked TBK1 deficiency in microglia induces an aged-like microglial signature and drives social recognition deficits in mice
    Article Snippet: Mice were placed on the Catwalk XT (Noldus), with wet paws to obtain robust prints despite no weight on the injured limb.



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    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were <t>FACS</t> sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.
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    Bio-Rad facs buffer
    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were <t>FACS</t> sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.
    Facs Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were FACS sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.

    Journal: bioRxiv

    Article Title: A Single-Cell Atlas of the Mouse Dural Meninges Reveals Pervasive Sex Differences Across Cellular Compartments

    doi: 10.64898/2026.05.12.721894

    Figure Lengend Snippet: A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were FACS sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.

    Article Snippet: The cell pellets were resuspended in ice-cold FACS buffer (0.1M PBS; 1mM EDTA (Fisher Scientific) and 1% BSA (Equitech-Bio; BAH70); pH 7.4).

    Techniques: Isolation, Expressing, Quantitative Proteomics